ABSTRACT
ObjectiveTo evaluate the pharmacodynamic effect of Huangqintang （HQT） on ulcerative colitis （UC） model mice and investigate its protective effect against UC by regulating intestinal flora. MethodMale Balb/c mice were randomly divided into control group，model group， high-， medium-， and low-dose HQT groups （20， 10， 5 g·kg-1）， flora interference group， flora interference model group， and flora interference-drug treatment group （HQT， 20 g·kg-1）. The flora interference model was constructed through intragastric administration of antibiotics （200 mg·kg-1 bacitracin and 200 mg·kg-1 vancomycin） for 8 d， and the UC model was constructed by allowing mice with free access to 3% dextran sulfate sodium （DSS） solution for 7 d. HQT was administered for 7 d. After the experiments， the mice were sacrificed， and blood， colon， and feces were collected. Hematoxylin-eosin （HE） staining was performed to observe the colonic lesions. The serum levels of interleukin （IL）-4， IL-6， IL-10， and tumor necrosis factor （TNF）-α were detected by enzyme-linked immunosorbent assay （ELISA）. The mRNA and protein expression of Claudin1， MUC1， Occludin， and zonula occludens-1（ZO-1） in colon tissues was detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） and Western blot， respectively. The fecal DNA of mice was extracted and analyzed by high-throughput sequencing. ResultCompared with the normal group， the model group showed increased serum content of IL-4， IL-6， and TNF-α （P<0.05， P<0.01） and decreased IL-10 （P<0.05）. Compared with the model group， the HQT groups displayed decreased serum levels of IL-4， IL-6， and TNF-α （P<0.05， P<0.01）， increased IL-10 content （P<0.01）， increased mRNA and protein expression levels of Claudin1， MUC1， Occludin， and ZO-1 （P<0.05， P<0.01）. After flora interference， the diversity and abundance of intestinal bacteria decreased. To be specific， Proteobacteria increased （P<0.01）， and Firmicutes and Bacteroidetes decreased （P<0.01）. After UC induction by DSS， Bacteroidetes and Tenericutes decreased （P<0.05）. The high-， medium-， and low-dose HQT groups showed increased Bacteroidetes and Tenericutes （P<0.05， P<0.01） and decreased Firmicutes （P<0.05）. Additionally， the abundance of Lactobacillus， Lachnospiraceae NK4A136 group， Escherichia-Shigella， and Helicobacteris was positively proportional to the dose of HQT. ConclusionHQT can inhibit the inflammatory response of UC mice， restore the imbalance of intestinal flora， and repair the damaged intestinal mucosal barrier.
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Objective To investigate the clinical efficacy of atorvastatin combined with aspirin in the treatment of acute cerebral infarction.Methods 160 patients with acute cerebral infarction were selected,they were randomly divided into two groups,80 cases in each group.The patients in both two groups used aspirin for treatment,while the observation group used atorvastatin for further treatment.All patients were followed up for 1 year,and the ADL score,the US National Institutes of Health neurological function (NIHSS) score,C-reactive protein (CRP),tumor necrosis factorα(TNF-α) and carotid intima-media thickness (IMT) were compared between the two groups.Results Six months and 12 months later after the treatment,the Barthel index in the observation group [(75.15 ± 6.67)points and (79.94 ± 7.84) points] were higher than those in the control group [(5.79 ± 0.68) points and (4.44 ±0.79) points],the differences were statistically significant (t =6.214,P =0.005;t =6.514,P =0.001).Six months and 12 months later after the treatment,the NIHSS scores in the observation group [(3.55 ± 0.85) points and (2.07 ±0.35) points]were higher than those in the control group[(5.79 ±0.68) points and (4.44 ±0.79) points],the differences were statistically significant(t =6.214,P =0.005;t =6.514,P =0.001).The levels of CRP,TNF-αand IMT of the observation group and the control group after treatment decreased significantly(the observation group:t =18.732,P =0.000;t =15.652,P =0.000;t =3.593,P =0.000;the control group:t =7.324,P =0.000;t =4.211,P =0.000;t =2.495,P =0.035).Moreover,the levels of CRP,TNF-α and IMT of the observation group were lower than those of the control group (t =8.893,P =0.000;t =5.819,P =0.000;t =2.984,P =0.015).Conclusion Atorvastatin combined with aspirin therapy can improve the daily skills and nerve function in patients with acute cerebral infarction.Moreover,it is effective to decrease the levels of CRP,TNF-α,IMT.
ABSTRACT
Objective To investigate the expression of the vascular endothelial growth factor (VEGF) and mucin MUC5AC in nasal mucosa before and after chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery.Methods 75 cases chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery patients were selected as nasal polyps group and 75 cases of nasal bone fracture or epistaxis patients as the control group from January 2012 to January 2015. Took the samples of nasal polyps before surgery and the maxillary sinus mucosa specimens after surgery six weeks of nasal polyps’ patients and on the edge of the inferior turbinate mucosa specimens of the control group to detect eosinophil count by HE staining, and detect the expression of VEGF and mucin MUC5AC by immunohistochemical staining.Results The specimens eosinophils of preoperative nasal polyp group and postoperative nasal polyp group were higher than that of control group (P < 0.05), the nasal eosinophils of postoperative nasal polyp group was lower than that of preoperative nasal polyp group (P < 0.05). The expression of specimens VEGF and mucin MUC5AC area percentages in preoperative nasal polyp group and postoperative nasal polyp group were higher than that in control group (P < 0.05), the expression of nasal VEGF and mucin MUC5AC area percentages in the postoperative nasal polyp group was lower than that of preoperative nasal polyp group (P< 0.05).Conclusion Eosinophil count and the expression levels of VEGF and mucin MUC5AC of nasal mucosa in chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery preoperative are higher, and reduce at postoperative six weeks, VEGF and mucin MUC5AC may be involved nasal repair.